DETECT IF P.carinii
P. carinii is ubiquitous, infecting man and other mammals; the route of infection is presumed to be airborne. It is a major pathogen in the immunocompromised, especially patients with AIDS where it is an established cause of pulmonary infection. Starvation, haematological malignancies, collagen vascular diseases, primary cellular immune deficiency and immunosuppressive therapy, for example in transplant patients and leukaemic patients on cytotoxic drugs are factors that increase the likelihood of infection with P. carinii pneumonia (PCP).
Currently, P. carinii pneumonia may be diagnosed by the observation of P. carinii in either open lung or transbronchial lung biopsy material, bronchoalveolar lavage or induced sputum. It can be visualised with a variety of non‑specific stains including Gomori methenamine silver, toluidine blue-O, Gram-Weigert, Giemsa and Wright‑Giemsa. Because all these stains react with yeasts and other structures, P. carinii must be distinguished on the basis of morphology. Staining techniques are time consuming and often require a high level of technical expertise in the interpretation of results. The Detect IF P. carinii test uses a murine monoclonal antibody reactive with both human and rodent P. carinii in a simple and rapid test for the detection and identification of P. carinii in human bronchoalveolar lavage fluid (BAL) and induced sputum (IS).
Part Number: FIPC200
Description: P.carinii monoclonal antibody, FITC-conjugated antibody, Enzyme and diluent, Hydrochloric acid, Specimen slides and mounting medium (5ml)
- Highly sensitive and specific assay
- Clearly defined results
- For use with both bronchoalveolar lavage and induced sputum specimens
- Control slides available
The Detect IF Pneumocystis carinii (P. carinii) test is an indirect qualitative immunofluorescence kit for the detection of P. carinii oocysts in human bronchoalveolar lavage fluid and induced sputum. It is intended to aid in the diagnosis of suspected P. carinii infection. Results should be interpreted in light of all clinical and diagnostic information.
Bronchoalveolar lavage fluid or pre-treated induced sputum specimens are centrifuged and washed. The pellets are resuspended, placed on slides and fixed. The specimens are Enzyme-digested. Murine anti-P. carinii antibody and fluorescently labelled anti-mouse antibody are added in turn after incubation, rinsing, wicking, and air-drying steps. On viewing with a fluorescence microscope, oocysts show as medium bright to bright apple green and may be evenly or unevenly labelled. The presence of P. carinii oocysts in bronchoalveolar lavage fluid or induced sputum indicates P. carinii infection.
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